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pbpA [2018-02-16 18:00:48]
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pbpA [2018-02-16 18:00:48]

class B penicillin-binding protein PBP 2A, required for cell wall synthesis during cell elongation
Locus
BSU25000
Isoelectric point
9.57
Molecular weight
79.96 kDa
Protein length
716 aa Sequence Blast
Gene length
2148 bp Sequence Blast
Function
formation of a rod-shaped peptidoglycan cell wall, spore outgrowth
Product
penicillin-binding protein PBP 2A
Essential
no
Synonyms
yqgF

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,581,771 → 2,583,921

The protein

Catalyzed reaction/ biological activity

  • required for cell wall synthesis during cell elongation PubMed
  • driver of MreB dynamics PubMed
  • Protein family

    Structure

  • 3VSK (from Staphylococcus aureus, 45% identity) PubMed
  • Localization

  • extracellular (signal peptide) PubMed
  • localization depends on the availability of peptidoglyan precursors (lipid II) PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
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    Biological materials

    Mutant

  • MGNA-C461 (yqgF::erm), available at the NBRP B. subtilis, Japan
  • BKE25000 (ΔpbpA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACATCACCTTTTCTAT, downstream forward: _UP4_TAAAAAAAAACGCTCACATG
  • BKK25000 (ΔpbpA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACATCACCTTTTCTAT, downstream forward: _UP4_TAAAAAAAAACGCTCACATG
  • GFP fusion

  • 3103 trpC2 pbpA::pSG5043 (cat Pxyl-gfpa-pbpA1-804) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab
  • Labs working on this gene/protein

  • Jeff Errington, Newcastle University, UK homepage
  • References

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